Available with Spatial Analyst license.
 A signature file is required when you use the geoprocessing tool Maximum Likelihood Classification to classify an image. To create a signature file, you can use the Create Signature File tool 
 on the Training Sample  Manager dialog box.
- Create the training samples using the Image Classification toolbar if they have not yet been created. See the Creating training samples step on how to create them.
 - Open Training Sample  Manager from the Image Classification toolbar. Click the Create Signature File button.

A file browser dialog box appears.
 - On the file browser dialog box, pick a location and specify a name for the signature file, then click OK to save the file.
 
A signature file is created and saved on disk.